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KMID : 0545120030130050828
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Journal of Microbiology and Biotechnology
2003 Volume.13 No. 5 p.828 ~ p.831
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Identification of 2-Deoxy-scyllo-inosose Synthase in Aminoglycoside Producer Streptomyces
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KHAREL, MADAN KUMAR
SUBBA, BIMALA/LEE, HEI CHAN/LIOU, KWANGKYOUNG/WOO, JIN SUK/KIM, DONG HWAN/MOON, YOUNG-HO
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Abstract
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Although most of the DOS containing aminoglycosides are produced by Streptomyces, very little information is available about their biosynthesis.
In the present paper. we report a method to isolate DO1 synthase, a key enzyme in the biosynthesis of DOS, from aminoglycoside producer Streptomyces. PCR primers based on conserved region of DO1 synthases were specific and reliable for the isolation of the biosynthetic genes of DOS containing aminoglycosides or the screening of the aminoglycoside producers. The use of DO1 synthase as a probe could save both time and cost of cloning aminoglycoside biosynthetic genes,
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KEYWORD
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